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Image Search Results
Journal: bioRxiv
Article Title: ALK signalling primes the DNA damage response sensitizing ALK-driven neuroblastoma to therapeutic ATR inhibition
doi: 10.1101/2023.08.30.555570
Figure Lengend Snippet: A. CLB-BAR and CLB-GE cell viability in response to increasing concentrations of elimusertib and ceralasertib. Data shown as mean ± SE of fold relative fluorescence units (RFU) relative to untreated cells from three independent experiments. B. ALK positive CLB-BAR and CLB-GE NB cells were treated for 24 h with elimusertib or ceralasertib as indicated. Cell lysates were immunoblotted for pATR (S428), ATR, pATM (S1981), ATM, pCHK1 (S345), CHK1, PARP/cl.PARP (quantified at right), p53 and pH2A.X (S139, quantified at right) antibodies. GAPDH was employed as loading control. C. CLB-GE cells were synchronized by thymidine block for 24 h followed by treatment with elimusertib or ceralasertib as indicated. Cells were stained for pFOXM1 (green) and DAPI (blue). At right, quantitation of cells positive for pFOXM1 (T600).
Article Snippet: Primary antibodies against ATR (#13934, 1:1000), pATR (S428, #2853, 1:1000), ATM (#2873, 1:1000), pATM (#13050, 1:1000), CHK1(#2345, 1:1000), pCHK1(S345,#2348, 1:1000), pCHK1(S280,#2347, 1:1000), pAKT (S473,#4060, 1:4000), DLG2 (#19046), pERK1/2 (Y204/T202, #4377, 1;2000), pALK (Y1278, #6941, 1:1000), β-Actin (#4970, 1:10000), AKT (#9272), FOXM1 (#20459, 1:1000),
Techniques: Fluorescence, Control, Blocking Assay, Staining, Quantitation Assay